Journal: Plants

Article Title: Identification of a GA-Related Cis -Element Regulating Male Peduncle Elongation in Papaya

doi: 10.3390/plants15020209

Figure Lengend Snippet: Histochemical staining of translational fusion constructs (designs in which a promoter drives the expression of a target gene coding sequence translationally fused to GUS, thereby producing a single fusion protein). Scale bars are 5 mm.

Article Snippet: Various tissues from T3 transformants (leaf, inflorescence, stem, and pedicels) were collected 44 days after planting, and incubated in GUS staining solution (0.1 M NaPO 4 at pH 7.0, 10 mM EDTA, 0.1% Triton X-100, 1 mM K 3 FE(CN) 6 [Thermo Scientific], 2 mM X-Gluc [Gold Biotechnology, St. Louis, MO, USA]) overnight.

Techniques: Staining, Construct, Expressing, Sequencing

Journal: Nature Communications

Article Title: AcRR1 of Agropyron cristatum boosts wheat yield by regulating grain number per spike and heading date

doi: 10.1038/s41467-025-61917-5

Figure Lengend Snippet: SEM image of young spikes ( a ) and central florets ( b ) from Fielder and AcRR1 -OE at the pistil and stamen formation stages. Scale bar, a 1 mm; b 0.5 mm. c Comparison of the florets number that eventually formed in Fielder and AcRR1 -OE. d GUS histochemical assays of Pro AcRR1 : GUS during young spike differentiation. I, floret differentiation stage; II, pistil and stamen formation stage; III, anther connective stage; IV, tetrad stage; V, white anther stage. Scale bar, 100 μm. In ( a ‒ d ) S, spikelet; F, floret primordia; AP, awn primordia; AF, apical floret; CF, central florets; BF, basal florets; A, anther. e , f Cytokinin and IAA contents of Fielder and AcRR1 -OE young spikes at the double ridge stage. g DEGs related to cytokinin degradation and biosynthesis in Fielder and AcRR1 -OE young spikes. h Expression of TaWOX11-2D in young spikes of AcRR1 -OE lines. i Direct binding of AcRR1 to the TaWOX11-2D promoter. j EMSA of the binding of AcRR1-His to the AGATN (A/T/C) motif in the TaWOX11-2D promoter. k ChIP‒qPCR assay of the binding of AcRR1 to the TaWOX11-2D promoter. l Representative spikes and middle spikelets of Jing 411 and the Tawox11-2D mutant. Scale bar, left, 2 cm; right, 1 cm. The grain number per spikelet ( m ) and grain number per spike ( n ) of F 2 TaWOX11-2D DD , TaWOX11-2D Dd and TaWOX11-2D dd plants derived from a cross of the Tawox11-2D mutant with Jing 411. o Direct binding of AcRR1 and wheat orthologs to the TaWOX11-2D promoter. The data are presented as the means ± S.D.s. In ( e ‒ i , k , o ) n = 3 biologically independent replicates. In ( c , m , n ) n represents the number of biologically independent plants. A two-tailed Student’s t test was used to determine P values. Source data are provided as a file.

Article Snippet: Histochemical analysis of GUS activity in Pro AcRR1 : GUS transgenic lines was performed with a GUS staining solution (Solarbio, China) according to the manufacturer’s instructions.

Techniques: Comparison, Expressing, Binding Assay, Mutagenesis, Derivative Assay, Two Tailed Test